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Primární osifikační centra dlouhých kostí se nacházejí v diafýze. Sekundární osifikační centra dlouhých kostí se nacházejí v epifýze. Kost je vytvářena činností osteoblastů, což jsou kostní buňky, které produkují organické látky tzv. kostního matrixu (kolagen, glykoproteiny ad.). Osteoklasty jsou též kostní buňky, ty však kostní tkáň odbourávají, „rozpouštějí“.
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